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جيل من الخلايا T CAR للعلاج بالتبني في سياق ورم أرومي دبقي مستوى الرعاية
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Generation of CAR T Cells for Adoptive Therapy in the Context of Glioblastoma Standard of Care

جيل من الخلايا T CAR للعلاج بالتبني في سياق ورم أرومي دبقي مستوى الرعاية

DOI:

12:55 min

February 16, 2015

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Kapitel

  • 00:05Titel
  • 01:59Administration of Temozolomide and Whole Brain Irradiation to Tumor-bearing Mice (days 0-4)
  • 03:26Cell Transfection, Splenectomy, and T Cell Preparation (day 0)
  • 06:09Transduction (days 1 and 2)
  • 08:10CAR T Cell and Administration to Tumor-bearing Mice (days 3-5)
  • 10:03Results: Antitumor Efficacy of CAR T Cells
  • 11:47Conclusion

Summary

Automatische Übersetzung

The lymphodepletive and immunomodulatory effects of chemotherapy and radiation standard of care can be leveraged to enhance the antitumor efficacy of T cell immunotherapy. We outline a method for generating EGFRvIII-specific chimeric antigen receptor (CAR) T cells and administering them in the context of glioblastoma standard of care.

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