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TIRFM과 pH 민감성 GFP - 프로브는 SH-SY5Y 신경 모세포종 세포에서 신경 전달 물질 소포 역학을 평가하기 : 세포 이미징 및 데이터 분석
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Kapitel
- 00:05Titel
- 03:32Cell Imaging by Total Internal Reflection Fluorescence Microscopy (TIRFM)
- 06:08Fluorescence Intensity Quantification
- 07:09Photobleaching Correction and Threshold Determination
- 08:16Selection of Fusion Events Using a Semiautomatic Procedure
- 10:01Data Analysis
- 11:31Results: Analysis of Synaptic Vesicles Fusion Under Resting and Stimulated (Potassium-induced Depolarization) Conditions Are Shown
- 12:37Conclusion
This paper provides a method for investigating neurotransmitter vesicle dynamics in neuroblastoma cells, using a synaptobrevin2-pHluorin construct and Total Internal Reflection Fluorescence Microscopy. The strategy developed for image processing and data analysis is also reported.
