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原代鼠脑微血管内皮细胞的分离
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Isolation of Primary Murine Brain Microvascular Endothelial Cells
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原代鼠脑微血管内皮细胞的分离

31,428 Views

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08:14 min

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November 14, 2014

DOI:

10.3791/52204-v

DOI:
10.3791/52204-v

•

08:14 min
November 14, 2014

•

67 Views
, , , ,
1Department of Neurology,University of Münster, 2Interdisciplinary Center for Clinical Research (IZKF) Münster, 3Institute of Physiology I — Neuropathophysiology I,University of Münster

Summary

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Brain microvascular endothelial cells (BMEC) are interconnected by specific junctional proteins forming a highly regulated barrier separating blood and the central nervous system (CNS), the so-called blood-brain-barrier (BBB). The isolation of primary murine brain microvascular endothelial cells, as discussed in this protocol, enables detailed in vitro studies of the BBB.

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Ruck, T., Bittner, S., Epping, L., Herrmann, A. M., Meuth, S. G. Isolation of Primary Murine Brain Microvascular Endothelial Cells. J. Vis. Exp. (93), e52204, doi:10.3791/52204 (2014).

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