Here, we describe a method for simultaneous quantification of T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs). The TREC/KREC assay can be used as marker of thymic and bone marrow output.
Sottini, A., Serana, F., Bertoli, D., Chiarini, M., Valotti, M., Vaglio Tessitore, M., Imberti, L. Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR. J. Vis. Exp. (94), e52184, doi:10.3791/52184 (2014).