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Quantificazione simultanea di T-Cell Receptor Excision Circles (TREC) e Circles K-Eliminazione ricombinazione Excision (KRECs) per Real-time PCR
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Quantificazione simultanea di T-Cell Receptor Excision Circles (TREC) e Circles K-Eliminazione ricombinazione Excision (KRECs) per Real-time PCR
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December 06, 2014
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Here, we describe a method for simultaneous quantification of T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs). The TREC/KREC assay can be used as marker of thymic and bone marrow output.
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Sottini, A., Serana, F., Bertoli, D., Chiarini, M., Valotti, M., Vaglio Tessitore, M., Imberti, L. Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR. J. Vis. Exp. (94), e52184, doi:10.3791/52184 (2014).
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