Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
網膜色素上皮細胞に貪食アッセイのためにブタまたはウシの感光体外側セグメントの大規模精製
11,697 Views
•
10:02 min
•
December 12, 2014
DOI:
Summary
Automatically generated
This article describes the protocol for the purification of photoreceptor outer segment fragments (POS) via ultracentrifugation from porcine/bovine retinae using homogenization and sucrose gradient centrifugation. This protocol allows the preparation of large stocks of POS aliquots, labeled or unlabeled, that can then be stored at -80 °C.
Read Article
Cite this Article
Parinot, C., Rieu, Q., Chatagnon, J., Finnemann, S. C., Nandrot, E. F. Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells. J. Vis. Exp. (94), e52100, doi:10.3791/52100 (2014).
Copy