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Kapitel
- 00:05Titel
- 01:09Polyethylenimine (PEI) Transfection
- 02:55ELISA
- 05:01Results: Detection of HIV-1 Env Expressed at the Surface of Transfected Cells by Monoclonal Antibodies
- 06:52Conclusion
Understanding viral surface antigens conformations is required to evaluate antibody neutralization and guide the design of effective vaccine immunogens. Here we describe a cell-based ELISA assay that allows the study of the recognition of trimeric HIV-1 Env expressed at the surface of transfected cells by specific anti-Env antibodies.
