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在实时的间皮细胞卵巢癌球体附件和入侵评估
JoVE Journal
Medizin
This content is Free Access.
JoVE Journal
Medizin
Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time
Please note that all translations are automatically generated.
Click here for the English version.
在实时的间皮细胞卵巢癌球体附件和入侵评估
DOI:
10.3791/51655-v
•
14:25 min
•
May 20, 2014
•
Maree Bilandzic
,
Kaye L. Stenvers
2
1
Reproductive Development and Cancer Laboratory
,
MIMR-PHI Institute of Medical Research
,
2
Department of Developmental Biology and Anatomy
,
Monash University
Kapitel
00:05
Titel
02:05
Optional Fluorescent Cell Labeling Method
03:24
Generation of Spheroids in Methylcellulose
04:28
Real Time Cell Analyzer (RTCA) Cell Invasion Assay
05:46
Programming the RTCA Instrument
07:25
Generation of LP9 Monolayer and Measurement of Spheroid Invasion
09:49
Results: Real Time Assessment of Ovarian Cancer Spheroid Invasion
13:42
Conclusion
Summary
Automatische Übersetzung
English (Original)
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Automatische Übersetzung
卵巢癌细胞侵入的腹膜间皮衬里是一个动态过程,随着时间的推移。利用实时分析器,卵巢癌细胞中的球状体,间皮细胞共培养模型的侵袭能力可以量化在延长的时间段,提供见解因子调控的转移过程。
Tags
Ovarian Cancer
Spheroid
Mesothelial Cells
Invasion
Real-time Analysis
Metastasis
Peritoneal Environment
Extracellular Matrix
Cell Lines
Quantitative Measurements
Article
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