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Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
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Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water

14,250 Views

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12:50 min

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June 09, 2014

DOI:

10.3791/51414-v

DOI:
10.3791/51414-v

•

12:50 min
June 09, 2014

•

2 Views
, , , , , , , , , ,
1Department of Animal Science,University of Wyoming, 2Department of Chemistry,Colorado State University, 3Department of Environmental and Radiological Health Sciences,Colorado State University, 4Department of Animal Sciences,Colorado State University, 5Department of Plant Sciences,University of California, Davis, 6Department of Food Science and Human Nutrition, Citrus Research and Education Center,University of Florida, 7Department of Food Science and Agricultural Chemistry,McGill University

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A protocol involving integrated concentration, enrichment, and end-point colorimetric detection of foodborne pathogens in large volumes of agricultural water is presented here. Water is filtered through Modified Moore Swabs (MMS), enriched with selective or non-selective media, and detection is performed using paper-based analytical devices (µPAD) imbedded with bacterial-indicative colorimetric substrates.

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Colorimetric DetectionPaper-based DetectionEscherichia ColiSalmonella Spp.Listeria MonocytogenesAgricultural WaterModified Moore SwabsEnrichmentPaper-based Analytical DevicesColorimetric SubstratesBacterial EnzymesMicrobiological Quality

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Bisha, B., Adkins, J. A., Jokerst, J. C., Chandler, J. C., Pérez-Méndez, A., Coleman, S. M., Sbodio, A. O., Suslow, T. V., Danyluk, M. D., Henry, C. S., Goodridge, L. D. Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water. J. Vis. Exp. (88), e51414, doi:10.3791/51414 (2014).

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