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生物层干涉测量的蛋白质 - 蛋白质相互作用和变构配体反应动力学的影响
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JoVE Journal Chemie
Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
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生物层干涉测量的蛋白质 - 蛋白质相互作用和变构配体反应动力学的影响

28,331 Views

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13:57 min

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February 18, 2014

DOI:

10.3791/51383-v

DOI:
10.3791/51383-v

•

13:57 min
February 18, 2014

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7 Views
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1Department of Biochemistry and Molecular Biology,SUNY Upstate Medical University

Tags

Protein-protein InteractionsAllosteric Ligand EffectsBio-layer InterferometryATP SynthaseSubunit εBacterial F-type ATP SynthaseConformational ChangeCatalytic ComplexInhibitory InteractionKineticsDrug Discovery

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Shah, N. B., Duncan, T. M. Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects. J. Vis. Exp. (84), e51383, doi:10.3791/51383 (2014).

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