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Imaging Plasma Membrane Deformations With pTIRFM
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April 02, 2014
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Polarization-based Total Internal Reflection Fluorescence Microscopy (pTIRFM) enables real-time detection of cell membrane dynamics. This article describes the implementation of pTIRFM for the study of membrane remodeling during regulated exocytosis. The technique is generalizable to other processes in cell biology that directly or indirectly involve changes in membrane shape.
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Passmore, D. R., Rao, T. C., Peleman, A. R., Anantharam, A. Imaging Plasma Membrane Deformations With pTIRFM. J. Vis. Exp. (86), e51334, doi:10.3791/51334 (2014).
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