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A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue
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A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue
Kapitel
- 00:05Titel
- 01:36hNPC Observations
- 02:13Chopper Setup
- 03:22Pre-chop Procedure
- 05:58Chop Procedure
- 07:08Post-chop Procedure
- 08:11Representative Results of hNPCs Aggregates Using a cGMP-applicable Expansion Method
- 09:04Conclusion
This protocol describes a novel mechanical chopping method that allows the expansion of spherical neural stem and progenitor cell aggregates without dissociation to a single cell suspension. Maintaining cell/cell contact allows rapid and stable growth for over 40 passages.
