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Measuring the Effects of Bacteria on C. Elegans Behavior Using an Egg Retention Assay
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Measuring the Effects of Bacteria on C. Elegans Behavior Using an Egg Retention Assay

Measuring the Effects of Bacteria on C. Elegans Behavior Using an Egg Retention Assay

DOI:
10.3791/51203-v

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08:36 min

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October 22, 2013

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, ,
1Department of Biological and Allied Health Sciences,Fairleigh Dickinson University

Chapters

  • 00:05Title
  • 01:22Preparation of Nematode Growth Plates
  • 02:44Seeding and Maintaining C. elegans
  • 04:32Preparation of rain Heart Infusion Plates for E. faecalis Culture
  • 05:26Egg in Worm Assay
  • 07:32Results: Effects on Pathogenic Bacteria on Egg Retention in C. elegans
  • 07:54Conclusion

Summary

Automatic Translation

Automatic Translation

An egg-in-worm (EIW) assay is a useful method to quantify egg-laying behavior. Alterations in egg laying can be a behavioral response of the model organism Caenorhabditis elegans to potentially harmful environmental substances such as those produced by pathogenic bacteria.

Tags

Egg-laying BehaviorC. ElegansEnterococcus FaecalisEgg-in-worm (EIW) AssayUterusEnvironmental CuesPathogenic BacteriaNeurotransmitter Signaling

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