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High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
Kapitel
- 00:05Titel
- 02:18Assay Set-up
- 04:00Incubation of Assay Plates
- 04:56Fluorescent Measurements on a Microplate Fluorometer
- 05:43Data Analysis
- 07:03Results: Analysis of Extracellular Enzyme Activity (EEA)
- 11:05Conclusion
To measure potential rates of soil extracellular enzyme activities, synthetic substrates that are bound to a fluorescent dye are added to soil samples. Enzyme activity is measured as the fluorescent dye is released from the substrate by an enzyme-catalyzed reaction, where higher fluorescence indicates more substrate degradation.
