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High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
JoVE 杂志
环境科学
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JoVE 杂志 环境科学
High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

45,133 Views

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12:33 min

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November 15, 2013

DOI:

10.3791/50961-v

DOI:
10.3791/50961-v

•

12:33 min
November 15, 2013

•

72 Views
, , , , ,
1Natural Resource Ecology Laboratory,Colorado State University, 2Biosciences Division,Oak Ridge National Laboratory, 3Department of Bioengineering,University of Colorado

Summary

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To measure potential rates of soil extracellular enzyme activities, synthetic substrates that are bound to a fluorescent dye are added to soil samples. Enzyme activity is measured as the fluorescent dye is released from the substrate by an enzyme-catalyzed reaction, where higher fluorescence indicates more substrate degradation.

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Cite this Article

Bell, C. W., Fricks, B. E., Rocca, J. D., Steinweg, J. M., McMahon, S. K., Wallenstein, M. D. High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities. J. Vis. Exp. (81), e50961, doi:10.3791/50961 (2013).

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