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Rescue of Recombinant Newcastle Disease Virus from cDNA
1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Global Health and Emerging Pathogens Institute,Icahn School of Medicine at Mount Sinai, 3Department of Medicine,Icahn School of Medicine at Mount Sinai, 4Department of Microbiology and Immunology, School of Medicine and Dentistry,University of Rochester
Kapitel
- 00:05Titel
- 01:37Infection of Mammalian Cells with the Recombinant Modified Vaccinia Ankara Virus Expressing the Bacteriophage T7 RNA Polymerase (MVA-T7)
- 02:40Transfection of Mammalian Cells
- 03:48Co-culture of Mammalian Cells with Avian Cells
- 05:22Infection of Chicken Embryonated Eggs
- 06:31Harvest of Allentoic Fluid from Infected Chicken Embryonated Eggs
- 07:52Hemagglutination (HA) Assay
- 09:03Results: Transfection and MVA-T7 Infection Efficiencies and Hemagglutination Assay
- 10:33Conclusion
Newcastle disease virus (NDV) has been extensively studied in the last few years in order to develop new vectors for vaccination and therapy, among others. These studies have been possible due to techniques to rescue recombinant virus from cDNA, such as those we describe here.
