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1Joint Unit Hospices de Lyon-bioMérieux, 2Medical Diagnostic Discovery Department,BioMérieux, 3Department of Pathology and Cytology, Centre Hospitalier Lyon Sud,Hospices Civils de Lyon, 4Medical Faculty,Lyon 1 University, 5Data and Knowledge Laboratory,BioMérieux, 6Department of Biochemistry and Molecular Biology, Centre Hospitalier Lyon Sud,Hospices Civils de Lyon, 7Department of Urology, Centre Hospitalier Lyon Sud,Hospices Civils de Lyon
Kapitel
- 00:05Titel
- 02:42Handling of Prostrate Tissues
- 04:03RNA Extraction, Purification, and Quality Control
- 05:53Target Preparation
- 07:33Hybridization to the HERV Chip Microarray
- 09:24Scanning and Data Analysis
- 10:14Results: Identification of Active HERV Loci in Prostrate Tissues
- 11:49Conclusion
Humane endogene retrovirus (Herv), som optager 8% af det menneskelige genom, fastholde knappe kodning kapaciteter, men hundrede tusinde lange terminale gentagelser (LTR). En brugerdefineret Affymetrix microarray var designet til at identificere individuelle HERV locus udtryk og blev brugt på prostatakræft væv som et proof of concept for fremtidige kliniske studier.
