This content is Free Access.
Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
1Joint Unit Hospices de Lyon-bioMérieux, 2Medical Diagnostic Discovery Department,BioMérieux, 3Department of Pathology and Cytology, Centre Hospitalier Lyon Sud,Hospices Civils de Lyon, 4Medical Faculty,Lyon 1 University, 5Data and Knowledge Laboratory,BioMérieux, 6Department of Biochemistry and Molecular Biology, Centre Hospitalier Lyon Sud,Hospices Civils de Lyon, 7Department of Urology, Centre Hospitalier Lyon Sud,Hospices Civils de Lyon
Kapitel
- 00:05Titel
- 02:42Handling of Prostrate Tissues
- 04:03RNA Extraction, Purification, and Quality Control
- 05:53Target Preparation
- 07:33Hybridization to the HERV Chip Microarray
- 09:24Scanning and Data Analysis
- 10:14Results: Identification of Active HERV Loci in Prostrate Tissues
- 11:49Conclusion
Human endogenous retroviruses (HERV), which occupy 8% of the human genome, retain scarce coding capacities but a hundred thousand long terminal repeats (LTRs). A custom Affymetrix microarray was designed to identify individual HERV locus expression and was used on prostate cancer tissues as a proof of concept for future clinical studies.
