Journal
/
Immunologie und Infektion
/
Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

DOI:
10.3791/50389-v

•

06:27 min

•

May 06, 2013

•

,
1Department of Microbiology & Immunology,Pennsylvania State University College of Medicine

Kapitel

  • 00:05Titel
  • 01:11Isolation of T Cells from Spleen and Lymph Nodes of BDC2.5 Mice
  • 02:52Fluorescence-activated Cell Sorting of Diabetogenic CD4+ T Cells from BDC2.5 Mice
  • 04:28Adoptive Transfer of Diabetogenic CD4+ T Cells from BDC2.5 Mice
  • 05:12Monitoring Recipient Mice for Hyperglycemia and T1D
  • 05:36Results: Adoptive T-cell Transfer
  • 06:10Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We provide a reproducible method to induce type 1 diabetes (T1D) in mice within two weeks by the adoptive transfer of islet antigen-specific, primary CD4+ T cells.

Tags

Accelerated Type 1 DiabetesAdoptive TransferDiabetogenic CD4+ T CellsNOD MouseAutoimmune DiabetesT Cell Receptor BDC2.5NOD.SCID RecipientT1D PathogenesisTherapeutic Interventions

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4

Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts

Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells

Read Article