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临床应用<em>睡美人</em和人工抗原呈递细胞基因修饰T细胞从外设和脐带血中
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood
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临床应用<em>睡美人</em和人工抗原呈递细胞基因修饰T细胞从外设和脐带血中

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09:29 min

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February 01, 2013

DOI:

10.3791/50070-v

DOI:
10.3791/50070-v

•

09:29 min
February 01, 2013

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, , , , , , , ,
1Division of Pediatrics,U.T. MD Anderson Cancer Center, 2Department of Stem Cell Transplantation and Cellular Therapy,U.T. MD Anderson Cancer Center

Summary

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T细胞表达CD19-特异性嵌合抗原受体（CAR）被注入我们的第一个人类基因治疗试验中研究治疗B细胞恶性肿瘤。我们描述了使用基因改造的T细胞<em>睡美人</em（SB）系统地介绍具体的CD19-CAR和选择性传播的名牌CD19 +人工抗原提呈细胞。

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Sleeping BeautyArtificial Antigen Presenting CellsGenetic ModificationT CellsPeripheral BloodUmbilical Cord BloodCAR T CellsCD19Gene TherapyImmunotherapyTumor-associated AntigensClinical Application

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Huls, M. H., Figliola, M. J., Dawson, M. J., Olivares, S., Kebriaei, P., Shpall, E. J., Champlin, R. E., Singh, H., Cooper, L. J. Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood. J. Vis. Exp. (72), e50070, doi:10.3791/50070 (2013).

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