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一个高含量的成像流程研究Grb2的信号复合体的表达克隆
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October 30, 2012
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高内涵筛选新的信号主管跨膜受体的鉴定方法。此方法是适合于大规模的自动化，并允许的预测<em在体内</em>蛋白结合和在哺乳动物细胞中的蛋白复合物的亚细胞定位。
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High-content ImagingGrb2 Signaling ComplexesExpression CloningSignal TransductionGrowth Factor ReceptorsDownstream Signaling CascadesMitogenic SignalingSH2 DomainSH3 DomainGTPase Son-Of-Sevenless (SOS)Phosphorylated Tyrosine ResiduesSOS-Ras-MAP Kinase Signaling CascadePositive And Negative Regulator Of SignalingReceptor EndocytosisMicroscopy AssayPlasma Membrane ReceptorsEpidermal Growth Factor Receptor (EGFR)GFP-Grb2
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Freeman, J., Kriston-Vizi, J., Seed, B., Ketteler, R. A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning. J. Vis. Exp. (68), e4382, doi:10.3791/4382 (2012).
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