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Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip
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Biotechnik
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JoVE Journal Biotechnik
Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip

Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip

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13:54 min

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July 07, 2012

DOI:

10.3791/4290-v

DOI:
10.3791/4290-v

•

13:54 min
July 07, 2012

•

13 Views
, , , , , ,
1Department of Plant Biology,Carnegie Institution for Science, 2Howard Hughes Medical Institute, 3Departments of Applied Physics and Bioengineering,Stanford University , 4Department of Microsystems Engineering (IMTEK) and Center for Biological Signaling Studies (BIOSS),University of Freiburg

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This article provides a protocol for cultivation of Arabidopsis seedlings in the RootChip, a microfluidic imaging platform that combines automated control of growth conditions with microscopic root monitoring and FRET-based measurement of intracellular metabolite levels.

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Cite this Article

Grossmann, G., Meier, M., Cartwright, H. N., Sosso, D., Quake, S. R., Ehrhardt, D. W., Frommer, W. B. Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip. J. Vis. Exp. (65), e4290, doi:10.3791/4290 (2012).

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