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Kapitel
- 00:05Titel
- 01:33Preparation and Set-up
- 02:56Collection of Embryos
- 04:38Preparation of Explants
- 06:49Migration of Cranial Mesenchyme Cells from Explants
- 07:21Conclusion
Cranial mesenchyme gjennomgår dramatiske morphogenic bevegelser som sannsynligvis gir en pådriver for heving av nevrale folder<sup> 1,2</sup>. Her beskriver vi en enkel<em> Ex vivo</em> Eksplanterer analyse for å karakterisere den cellulære atferd av cranial mesenchyme under neurulation. Denne analysen har mange bruksområder, inkludert å være mottagelig for farmakologiske manipulasjoner og sanntidsavbildning analyser.
Tags
Explant AssayCellular BehaviorCranial MesenchymeCentral Nervous SystemNeural PlateNeurulationMorphogenetic MovementsNeural Tube FormationParaxial MesodermNeural Crest CellsPorous MeshworkStellate Shaped CellsExtracellular Matrix (ECM) StrandsStereotypical RearrangementsExpansionDriving ForcePathwaysCellular BehaviorsInteractions Between ECM And CellsEx Vivo AssayPharmacological ManipulationsSignaling PathwaysLive Imaging AnalysesMigratory Properties
