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Kapitel
- 00:05Titel
- 01:33Preparation and Set-up
- 02:56Collection of Embryos
- 04:38Preparation of Explants
- 06:49Migration of Cranial Mesenchyme Cells from Explants
- 07:21Conclusion
Den kraniel mesenkym undergår dramatiske morfogene bevægelser, der sandsynligvis giver en drivkraft for elevation af de neurale folder<sup> 1,2</sup>. Her beskriver vi en enkel<em> Ex vivo</em> Eksplanteres assay til at karakterisere de cellulære adfærd af den kranielle mesenkym under neurulation. Denne analyse har talrige anvendelser, herunder at være modtagelige for farmakologiske manipulationer og levende billeddiagnostiske analyser.
Tags
Explant AssayCellular BehaviorCranial MesenchymeCentral Nervous SystemNeural PlateNeurulationMorphogenetic MovementsNeural Tube FormationParaxial MesodermNeural Crest CellsPorous MeshworkStellate Shaped CellsExtracellular Matrix (ECM) StrandsStereotypical RearrangementsExpansionDriving ForcePathwaysCellular BehaviorsInteractions Between ECM And CellsEx Vivo AssayPharmacological ManipulationsSignaling PathwaysLive Imaging AnalysesMigratory Properties
