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An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
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An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
Kapitel
- 00:05Titel
- 01:05Preparing Injection Needles and Embryos
- 02:22Injecting Brain Ventricles
- 03:22Image and Quantification of Dye Movement
- 04:44Representative Permeability Results of Neuroepithelium
- 06:18Conclusion
We describe a live whole animal quantitative measurement for permeability of the embryonic zebrafish brain. The technique analyzes the ability to retain cerebrospinal fluid and molecules of different molecular weights within the neural tube lumen and quantifies their movement out of the ventricles. This method is useful for determining differences in epithelial permeability and maturation during development and disease.
