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Electricity-Free, Sequential Nucleic Acid and Protein Isolation
Kapitel
- 00:05Titel
- 02:20Sample Lysis
- 03:20Nuclei Acid Extraction
- 05:27Protein Extraction
- 07:11Results: Sequential Nucleic Acid and Protein Isolation
- 09:28Conclusion
A tool and chemistries are described to sequentially isolate nucleic acids followed by proteins from a sample without the need for electricity. The tool consists of a sorbent held within a transfer pipette while the isolation chemistries are based on solid-phase extraction principles. The isolated macromolecules can be analyzed by immuno-based and PCR-based assays.
Tags
Electricity-freeSequentialNucleic Acid IsolationProtein IsolationTraditional PathogensEmerging PathogensEnterohemorrhagic Escherichia Coli (EHEC)Yersinia PestisPrion-based DiseasesConcernGovernmentsIndustriesMedical ProfessionalsDeathsChildrenDeveloping WorldLaboratory-based IdentificationUnited StatesLow CostField-based ToolsPoint-of-careRapid IdentificationDiagnosisDisease MarkersDisease ProgressionPatient PrognosisSolid-phase Extraction (SPE)Nucleic AcidsProteinsSample Isolation ToolBOOM TechnologyChaotropic Salt Solution
