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Biologie
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高通量的亲和标签的重组蛋白的纯化
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal
Biologie
High-throughput Purification of Affinity-tagged Recombinant Proteins
Please note that all translations are automatically generated.
Click here for the English version.
高通量的亲和标签的重组蛋白的纯化
DOI:
10.3791/4110-v
•
07:44 min
•
August 26, 2012
•
Simone C. Wiesler
,
Robert O.J. Weinzierl
1
Department of Life Sciences
,
Imperial College London
Kapitel
00:05
Titel
01:27
High-throughput Growth of Bacterial Cultures
02:38
Robotic Purification of Recombinant Proteins
04:06
Protein Purification
06:32
Representative Protein Purification Results
07:08
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
我们描述了一种方法,亲和标签的重组蛋白的纯化,使用的液体处理机器人。此方法通常是适用于小规模纯化的可溶性的带有His标记的蛋白质在高吞吐量的格式。
Tags
High-throughput Purification
Affinity-tagged Recombinant Proteins
X-ray Crystallography
Biochemical Analyses
Structure/function Relationships
Large-scale Mutagenesis Strategies
Amino Acids Substitution
Gain/loss-of-function Phenotypes
Catalytic Activity
Protein Stability
Protein-protein Interaction Specificity
Controlled And Reproducible Manner
High-throughput Strategies
Robotic Liquid-handling Platforms
His-tagged Recombinant Proteins
Basal Transcription Machinery
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