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June 16, 2012
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在这份报告中，我们描述了一个隔离高度纯化的白细胞浸润肿瘤人口的协议。该协议是改编自美天旎生物技术协议，以提高产量和隔离从复杂的肿瘤组织细胞的纯度。
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IsolationImmune CellsPrimary TumorsImmunosuppressive MicroenvironmentLeukocytesChemokinesGrowth FactorsTumor-associated LeukocytesTumor-draining Lymph NodesSpleenCell ActivationTraffickingDendritic CellsPhenotypeAdoptively-transferred T CellsTolerizedProliferationCytokine ProductionTumor MicroenvironmentCD4+ T Helper CellsCD8+ T Cells
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Watkins, S. K., Zhu, Z., Watkins, K. E., Hurwitz, A. A. Isolation of Immune Cells from Primary Tumors. J. Vis. Exp. (64), e3952, doi:10.3791/3952 (2012).
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