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Isolation and Genome Analysis of Single Virions using ‘Single Virus Genomics’
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Isolation and Genome Analysis of Single Virions using ‘Single Virus Genomics’

Isolation and Genome Analysis of Single Virions using ‘Single Virus Genomics’

DOI:
10.3791/3899-v

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08:31 min

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May 26, 2013

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, , , , ,
1Department of Microbial and Environmental Genomics,The J. Craig Venter Institute

Kapitel

  • 00:05Titel
  • 02:11Flow Cytometry
  • 03:54Visualizing Single Virions using Confocal Microscopy and Whole Genome Amplification
  • 06:34Results: Representative Isolation and Amplification of a Single Viral Genome
  • 07:54Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Single Virus Genomics (SVG) is a method to isolate and amplify the genomes of single virons. Viral suspensions of a mixed assemblage are sorted using flow cytometry onto a microscope slide with discrete wells containing agarose, thereby capturing the virion and reducing genome shearing during downstream processing. Whole genome amplification is achieved using multiple displacement amplification (MDA) resulting in genomic material that is suitable for sequencing.

Tags

IsolationGenome AnalysisSingle VirionsSingle Virus GenomicsWhole Genome AmplificationSequencingSingle Microbial CellsGenomic CharacterizationCultivationVirusesUbiquitousNumerous EntitiesEnvironmentsIsolatingCharacterizing GenomesFlow CytometryHigh Molecular Weight Genomic DNA

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