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Isolamento e Cultura de neurônios do hipocampo de ratos pré-natal
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July 26, 2012
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Nós fornecer um protocolo para a cultura de neurónios do hipocampo altamente purificado a partir de cérebros de rato pré-natal, sem a utilização de uma camada de células alimentador glial.
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IsolationCultureHippocampal NeuronsPrenatal MicePrimary CulturesRatMurineNeurobiologyCellular MechanismsCellular TraffickingCellular StructureProtein LocalizationBiochemical TechniquesMemoryLearningContaminationBrain Cell TypesSpecific MediaNeuron GrowthDissection TechniquesEmbryonic Day 19 (E19)Mouse PupsTrypsinMechanical DisruptionPipettes
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Seibenhener, M. L., Wooten, M. W. Isolation and Culture of Hippocampal Neurons from Prenatal Mice. J. Vis. Exp. (65), e3634, doi:10.3791/3634 (2012).
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