Journal
/
Immunologie und Infektion
/
Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus

Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus

DOI:
10.3791/3627-v

•

11:18 min

•

May 07, 2012

•

, , ,
1Center for Cell and Gene Therapy,Baylor College of Medicine , 2Pathology and Immunology,Baylor College of Medicine , 3Department of Stem Cell Transplantation and Cellular Therapy,University of Texas M.D. Anderson Cancer Center, 4Medizin,Baylor College of Medicine , 5Department of Pediatrics,Baylor College of Medicine

Kapitel

  • 00:05Titel
  • 02:14Mononuclear Cell Isolation
  • 03:53Dendritic Cell (DC) Generation
  • 05:35Generation of EBV-LCL
  • 06:20CTL Initiation
  • 09:22Representative Results
  • 10:34Conclusions

Summary

Automatische Übersetzung

Automatische Übersetzung

Here we describe the first good manufacturing practice (GMP)-compliant method of producing virus-specific cytotoxic T lymphocytes (CTL) from umbilical cord blood, a source of predominantly naîve T cells.

Tags

Cytotoxic T LymphocytesUmbilical Cord BloodCytomegalovirusEpstein-Barr VirusAdenovirusStem Cell TransplantationCord Blood TransplantationVirus-specific CTLDendritic CellsAdenoviral VectorCMV Antigen Pp65IL-7IL-12IL-15EBV-transformed B CellsEBV-LCL

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

check_url/de/3627?article_type=v

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays
check_url/de/3627?article_type=v

Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes
check_url/de/3627?article_type=v

Introducing Shear Stress in the Study of Bacterial Adhesion
check_url/de/3627?article_type=v

Etablering av Epstein-Barr Virus Vekst-forvandlet lymfoblastoid Cell Lines
check_url/de/3627?article_type=v

Klinisk anvendelse av<em> Sleeping Beauty</em> Og kunstig antigenpresenterende celler å genmodifisere T celler fra perifert og navlestrengsblod
check_url/de/3627?article_type=v

Isolering av forløper B-celle undergrupper fra navlestrengsblod
check_url/de/3627?article_type=v

Isolasjon og Th17 Differensiering av naive CD4 T-lymfocytter
check_url/de/3627?article_type=v

Utvikling av en IFN-γ ELISPOT analysen å vurdere varicella-zoster virus-spesifikke Cell-mediert immunitet Etter Umbilical Cord Blood Transplantasjon
check_url/de/3627?article_type=v

Generation of Human alloantigen spesifikke T-celler fra perifert blod
check_url/de/3627?article_type=v

Radial Mobilitet og Cytotoksisk Funksjon av Retroviral Replikere Vector transduserte, Ikke-tilhenger Alloresponsive T-lymfocytter

Read Article