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Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents
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JoVE Journal Neurowissenschaften
Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

DOI:
10.3791/3568-v

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17:37 min

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March 04, 2012

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, , , , , , , , , , , , , ,
1Center for Molecular and Behavioral Neuroscience,University of New Jersey, 2Center for Interdisciplinary Research in Biology,Collège de France, 3Janelia Farm Research Campus,Howards Hughes Medical Institute, 4Deptartment of Psychology,University of Wisconsin at Milwaukee

Kapitel

  • 00:02Titel
  • 01:13Construction of the microdrive
  • 04:25Preparing the silicon probe
  • 05:55Affixing the probe to the microdrive
  • 06:37Preparing the skull
  • 10:53Preparing the brain surface
  • 12:40Implanting the probe
  • 14:07Building the on-head Faraday cage
  • 15:49Recording from the freely moving animal
  • 17:00Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We describe methods for large-scale recording of multiple single units and local field potential in behaving rodents with silicon probes. Drive fabrication, probe attachment to the drive and probe implantation processes are illustrated in sufficient details for easy replication.

Tags

NeurowissenschaftenLarge-scale RecordingMovable Silicon ProbesBehaving RodentsNeural AssembliesInput-output RelationshipsSpatial SelectivityTemporal ResolutionMechanistic AnalysisNeural EnsemblesMultiple-site RecordingSilicon-based DevicesMicro-machined Silicon ProbesCustom-made Accessory ComponentsPreamplifiersPrinted Circuit BoardsFlexible CablesBonding ServiceImplantation4-shank Probe32-site ProbePolyimide CableMovable Microdrive

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