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1Department of Biomedical Engineering,University of Wisconsin-Madison, 2Materials Science Program,University of Wisconsin-Madison, 3Department of Neurological Surgery,University of Wisconsin-Madison, 4Carbone Comprehensive Cancer Center and Center for Stem Cell and Regenerative Medicine,University of Wisconsin-Madison
Kapitel
- 00:05Titel
- 01:57BTSC Neurosphere Dissociation
- 03:00Fabrication of Bi-layered su-8 Master
- 04:42Molding of the PDMS Stamp
- 05:31Assembly of Microfluidic Devices and Cell Culturing
- 07:04Long-term Time-lapse Imaging of BTSC Migration with BioStation IM
- 07:46Time-lapse Recordings of Migrating BTSC
- 08:40Conclusion
Kanser kök hücre göçü araştırılması için bir bölme oluşturan mikroakışkan cihaz tarif edilmektedir. Bu yeni platform canlı hücresel mikroçevrede oluşturur ve canlı hücre lokomosyon mikroskobik görselleştirme sağlar. Son derece hareketli kanser hücrelerinin potansiyel olarak daha etkin gelecekteki tedaviler neden agresif infiltrasyon moleküler mekanizmaları, çalışma izole edilmiştir.
Tags
Cancer Stem Cell MigrationCompartmentalizing Microfluidic DevicesLive Cell ImagingKrebsforschungCellular MigrationAggressive Cancer Cell InvasionMetastasisTherapeutic ResistanceGlioblastoma Multiforme (GBM)Primary Malignant Adult Brain TumorGBM InfiltrationTumor MicroenvironmentCancer Cell Migration MechanismsBrain Tumor Stem Cell (BTSC)Therapeutic ResistanceRecurrenceCell Migratory MechanismsConventional Migration AssaysMicrofluidic DevicesSingle Cell Analysis
