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癌症干细胞迁移的使用进行划分的微流控芯片和活细胞成像的评价
JoVE Journal
Medizin
This content is Free Access.
JoVE Journal
Medizin
Evaluation of Cancer Stem Cell Migration Using Compartmentalizing Microfluidic Devices and Live Cell Imaging
Please note that all translations are automatically generated.
Click here for the English version.
癌症干细胞迁移的使用进行划分的微流控芯片和活细胞成像的评价
DOI:
10.3791/3297-v
•
09:36 min
•
December 23, 2011
•
Yu Huang*
1,2
,
Basheal Agrawal*
3
,
Paul A. Clark
,
Justin C. Williams
2,3
,
John S. Kuo
4
1
Department of Biomedical Engineering
,
University of Wisconsin-Madison
,
2
Materials Science Program
,
University of Wisconsin-Madison
,
3
Department of Neurological Surgery
,
University of Wisconsin-Madison
,
4
Carbone Comprehensive Cancer Center and Center for Stem Cell and Regenerative Medicine
,
University of Wisconsin-Madison
Kapitel
00:05
Titel
01:57
BTSC Neurosphere Dissociation
03:00
Fabrication of Bi-layered su-8 Master
04:42
Molding of the PDMS Stamp
05:31
Assembly of Microfluidic Devices and Cell Culturing
07:04
Long-term Time-lapse Imaging of BTSC Migration with BioStation IM
07:46
Time-lapse Recordings of Migrating BTSC
08:40
Conclusion
Summary
Automatische Übersetzung
English (Original)
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Automatische Übersetzung
一个调查癌症干细胞迁移的微流体装置进行划分。这个新的平台创建一个可行的细胞微环境,使活细胞运动的微观可视化。高度能动的癌症细胞分离分子机制研究的积极渗透,并可能导致未来更有效的疗法。
Tags
Cancer Stem Cell Migration
Compartmentalizing Microfluidic Devices
Live Cell Imaging
Krebsforschung
Cellular Migration
Aggressive Cancer Cell Invasion
Metastasis
Therapeutic Resistance
Glioblastoma Multiforme (GBM)
Primary Malignant Adult Brain Tumor
GBM Infiltration
Tumor Microenvironment
Cancer Cell Migration Mechanisms
Brain Tumor Stem Cell (BTSC)
Therapeutic Resistance
Recurrence
Cell Migratory Mechanisms
Conventional Migration Assays
Microfluidic Devices
Single Cell Analysis
Article
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