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Journal
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Immunologie und Infektion
/
RNA的分离
绿脓杆菌
殖民小鼠胃肠道
JoVE Journal
Immunologie und Infektion
This content is Free Access.
JoVE Journal
Immunologie und Infektion
RNA Isolation of
Pseudomonas aeruginosa
Colonizing the Murine Gastrointestinal Tract
Please note that all translations are automatically generated.
Click here for the English version.
RNA的分离
绿脓杆菌
殖民小鼠胃肠道
DOI:
10.3791/3293-v
•
08:52 min
•
September 28, 2011
•
Eduardo Lopez-Medina
,
Megan M. Neubauer
,
Gerald B. Pier
,
Andrew Y. Koh
1
Department of Pediatrics
,
University of Texas Southwestern Medical Center
,
2
Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital
,
Harvard Medical School
,
3
Department of Pediatrics and Microbiology
,
University of Texas Southwestern Medical Center
Kapitel
00:05
Titel
01:21
Harvesting Murine Luminal Contents
02:44
Bacterial RNA Isolation
06:03
DNAse Treatment
07:48
Representative Agilent Biolanalyzer Analysis of Murine Cecal Content-extracted Bacterial RNA
08:35
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
RNA提取的一种可靠的方法<em>绿脓杆菌</em从小鼠cecums恢复描述。回收的RNA是足够的数量和质量,为后续的qPCR,转录分析和RNA序列的实验。这种技术可以适用于RNA等肠道微生物的隔离。
Tags
RNA Isolation
Pseudomonas Aeruginosa
Gastrointestinal Tract
Colonization
Immune Systems
Leukemia
Burn Wounds
Organ Transplants
Bloodstream Infections
Transcription Profiling
MRNA
Gene Expression
Immune Status
Isolation
High Quality MRNA
Enzymes
Debris
Food Residues
Particulate Matter
GI Colonization
Neutropenia-induced Dissemination
Mice Euthanization
Article
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共培养模型
绿脓杆菌
现场人类呼吸道细胞生长的生物膜
铜绿假单胞菌
和
酿酒酵母
流细胞生物膜
长期慢性<em>铜绿假单胞菌</em>在呼吸道感染小鼠
绿脓杆菌
引起的肺损伤模型
来到光明的一面
: 体内
糖尿病无毛小鼠慢性伤口生物膜感染的监测
复制的有序, 非冗余图书馆的
铜绿假单
胞菌应变 PA14 座子插入突变体
铜绿
假单胞
菌感染肺内皮细胞后细胞毒性 Amyloids 的检测方法
使用荧光原位杂交对毛尿胃肠道中的
念珠菌
的可视化
伪多蒙多尼亚
小殖民地文化及其藻酸盐的定量
慢性
假单位
病伤口感染的延迟接种模型
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