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Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping
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Biologie
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JoVE Journal Biologie
Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping

Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping

22,531 Views

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11:15 min

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November 09, 2011

DOI:

10.3791/3280-v

DOI:
10.3791/3280-v

•

11:15 min
November 09, 2011

•

2 Views
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1Horticulture Department,University of Wisconsin-Madison, 2Department of Zoology,Oregon State University

Summary

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The Ice-Cap method allows one to grow plants in 96-well plates and non-destructively harvest root tissue from each seedling. DNA extracted from this root tissue can be used for genotyping reactions. We have found that Ice-Cap works well for Arabidopsis thaliana, tomato, and rice seedlings.

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Cite this Article

Su, S., Clark, K. A., Gibbs, N. M., Bush, S. M., Krysan, P. J. Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping. J. Vis. Exp. (57), e3280, doi:10.3791/3280 (2011).

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