/
/
Icke-Laser Capture Mikroskopi metoden för Microdissection diskreta Regioner Mouse Brain för Total RNA Isolering och nedströms nästa generations sekvensering och Gene expression profiling
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Icke-Laser Capture Mikroskopi metoden för Microdissection diskreta Regioner Mouse Brain för Total RNA Isolering och nedströms nästa generations sekvensering och Gene expression profiling
Kapitel
- 00:05Titel
- 01:40Sampling Setup and Preparation
- 04:30Brain Removal and Coronal Sectioning
- 06:05Brain Region Microdissection and Tissue Archival
- 07:44Total RNA Extraction
- 09:01Representative RNA Isolation Results
- 09:38Conclusion
RNA expression profiling of discrete mouse brain regions requires a precise and repeatable tissue collection strategy. A protocol that uses both coronal brain sectioning and tissue corer-assisted microdissection is described here. The yield and quality of total RNA obtained from the resulting samples confirms the utility of the outlined method.
Tags
Non-laser Capture MicroscopyMicrodissectionMouse Brain RegionsTotal RNA IsolationNext-generation SequencingGene Expression ProfilingTechnological PlatformsNext-generation SequencingMicroarrayQRT-PCRHigh-resolution RNA SequencingRNA-SeqTranscript LevelsAlternative SplicingMicro RNAsWhole Transcriptome ProfilingPhenotypically Distinct GroupsMolecular MechanismsPhysiological StatesDisease ProgressionNext-generation Sequencing IntegrationGene Expression StudiesMouse ModelsBrain Nuclei PrecisionLaser Capture Microscopy (LCM)Sample Excision
