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10:45 min
October 13, 2011
DOI:
10.3791/3089-v
描述的方法,用于制备一个3 - 维的矩阵组成的I型胶原蛋白和初级人成纤维细胞。器官凝胶作为一个有用的基体,以评估侵入细胞的迁移,因为它模仿组织基质的基本功能,是适合各种形式的显微镜。
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Timpson, P., Mcghee, E. J., Erami, Z., Nobis, M., Quinn, J. A., Edward, M., Anderson, K. I. Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context. J. Vis. Exp. (56), e3089, doi:10.3791/3089 (2011).
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