/
/
Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context
This content is Free Access.
Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context
22,074 Views
•
10:45 min
•
October 13, 2011
DOI:
Summary
Automatically generated
A method is described for the preparation of a 3-dimensional matrix consisting of collagen type I and primary human fibroblasts. This organotypic gel serves as a useful substrate to assess invasive cell migration because it mimics basic features of tissue stroma and is amenable to many forms of microscopy.
Explore More Videos
Organotypic Collagen I AssayCell Behavior3-dimensional ContextCell MigrationBiologieDevelopmentWound HealingImmune SystemTumor CellsGlass CoverslipsLight MicroscopyLocal EnvironmentElasticityProtein CompositionPore SizeTwo-dimensional SubstratesStromal FibroblastsImmune CellsInvasion Of Cancer CellsMolecular DynamicsDrug Treatmentresponse To Drug TreatmentIn Vitro And In Vivo ComparisonCell Derived MatrixMatrigelOrganotypic Culture
Read Article
Cite this Article
Timpson, P., Mcghee, E. J., Erami, Z., Nobis, M., Quinn, J. A., Edward, M., Anderson, K. I. Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context. J. Vis. Exp. (56), e3089, doi:10.3791/3089 (2011).
Copy