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Measuring the Kinetics of mRNA Transcription in Single Living Cells
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JoVE Journal Biologie
Measuring the Kinetics of mRNA Transcription in Single Living Cells

Measuring the Kinetics of mRNA Transcription in Single Living Cells

DOI:
10.3791/2898-v

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11:22 min

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August 25, 2011

•

,
1The Mina and Everard Goodman Faculty of Life Sciences and Institute of Nanotechnology,Bar-Ilan University

Kapitel

  • 00:05Titel
  • 02:33Tagging of mRNA in Living Cells
  • 04:55FRAP Experiment
  • 08:10Data Analysis
  • 09:44Results of Measuring mRNA Transcription Kinetics
  • 10:51Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

RNA polymerase II transcriptional kinetics are measured on specific genes in living cells. mRNAs transcribed from the gene of interest are fluorescently tagged and using Fluorescence Recovery After Photobleaching (FRAP) the in vivo kinetics of transcriptional elongation are obtained.

Tags

KineticsMRNA TranscriptionLiving CellsRNA Polymerase IITranscriptional ActivityVisualization MethodsAnalysisElongation KineticsGene LocusFluorescent LabelingMS2 SystemMRNA TranscriptsYFP-MS2 Coat ProteinFluorescence Recovery After Photobleaching (FRAP) MethodSynthesis Rate

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