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Estrazione DNA da materiale in paraffina per le analisi genetiche ed epigenetiche
JoVE Journal
Biologie
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JoVE Journal Biologie
DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
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Estrazione DNA da materiale in paraffina per le analisi genetiche ed epigenetiche

55,346 Views

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13:32 min

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March 26, 2011

DOI:

10.3791/2763-v

DOI:
10.3791/2763-v

•

13:32 min
March 26, 2011

•

28 Views
, , ,
1Department of Integrative Oncology,BC Cancer Research Centre, 2Interdisciplinary Oncology Program,University of British Columbia – UBC, 3Photography/Video Production, Multi-Media Services,BC Cancer Agency, 4Department of Pathology and Laboratory Medicine,University of British Columbia – UBC

Summary

Automatically generated

Questo video dimostra il protocollo per l'estrazione del DNA da fissati in formalina materiale in paraffina. Questa è una di più giorni procedura di sezioni di tessuto in cui sono deparaffinate con xilene, reidratato con etanolo e trattati con proteinasi K per purificare e isolare il DNA per le successive analisi del gene-specifici o genoma.

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Cite this Article

Pikor, L. A., Enfield, K. S. S., Cameron, H., Lam, W. L. DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses. J. Vis. Exp. (49), e2763, doi:10.3791/2763 (2011).

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