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Vibrodissociation从啮齿动物脑切片的神经元，研究突触传递和图像突触前的码头
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May 25, 2011
DOI:
1Section on Synaptic Pharmacology/Laboratory for Integrative Neuroscience,National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism, 2Department of Electronics Engineering,Ewha Womans University, 3Section on Transmitter Signaling/Laboratory of Molecular Physiology,National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism
Summary
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这份报告表明保留个别可行的神经元连接的突触前boutons的机械隔离技术。 Vibrodissociated的神经元具有快速生产的优势，卓越的药理控制和改进的空间不影响邻近的细胞钳。这种方法可用于突触元素的成像和膜片钳记录。
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Cite this Article
Jun, S. B., Cuzon Carlson, V., Ikeda, S., Lovinger, D. Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals. J. Vis. Exp. (51), e2752, doi:10.3791/2752 (2011).
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