Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Use of Fluorescent Immuno-Chemistry for the…
JoVE Journal
Immunology and Infection

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Immunology and Infection
Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples
 

Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples

Article DOI: 10.3791/2687-v • 05:24 min • May 10th, 2011
May 10th, 2011 •
1, 1
1Department of Basic Sciences, Mississippi State University

Kapitel

Summary
Automatic Translation

Here we describe a procedure allowing the labeling of Edwardsiella ictaluri in situ in histological sections from channel catfish Ictalurus punctatus using indirect immunohistochemistry with monoclonal antibodies Ed9 as a primary, and fluorescent FitC labeled antibodies as a secondary. This allowed for the detection of the bacterium using fluorescent microscopy.

Tags

Fluorescent Immuno-Chemistry Detection Edwardsiella Ictaluri Channel Catfish Ictalurus Punctatus Method Development In Situ Visualization Bacteria Histological Sections Bacterial Localization Plate Counts Radiometric Labeled Bioluminescent Bacteria Gross Organ Level Infection Cycle Virulence Factors Interaction With Host Salmonella Typhi Taxonomic Family Technique Description Indirect Immuno-histochemistry Monoclonal Ed9 Antibody Blocking Serum Paraffin Embedded Histological Sections Primary Antibody Secondary Antibodies FitC Labeled Secondary Antibodies
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter