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DNA Microarrays: Voorbeeld Quality Control, Array Hybridisatie en scannen
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March 15, 2011
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We demonstreren het gebruik van DNA-microarrays voor expressie profilering van het zenuwstelsel. We beschrijven RNA kwaliteitscontrole, monster etikettering, en array-hybridisatie en scannen.
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DNA MicroarraysSample Quality ControlArray HybridizationScanningGene ActivitiesNervous SystemDevelopment StagesPhysiological StatesPathological StatesTherapy ResponseExperimental TestingHigh Quality RNAAmplificationHybridization ProtocolsAgilent’s 2100 BioanalyzerLab-on-a-chip TechnologyReverse TranscriptionCDNAIn Vitro TranscriptionLabeled CRNADual-color Labeling KitCy3Cy5Agilent’s 4×44 K Arrays
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Diaz, E., Barisone, G. A. DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning. J. Vis. Exp. (49), e2546, doi:10.3791/2546 (2011).
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