Journal
/
Biologie
/
DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal Biologie
DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

DOI:
10.3791/2546-v

•

09:27 min

•

March 15, 2011

•

,
1Department of Pharmacology,University of California, Davis

Kapitel

  • 00:05Titel
  • 00:51Quality Control Analysis of RNA Sample with 2100 Bioanalyzer
  • 03:05Amplification and Labeling
  • 03:44Microarray Hybridization
  • 05:52Microarray Washing
  • 07:30DNA Microarray Representative Results
  • 09:08Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We demonstrate the use of DNA microarrays for expression profiling of the nervous system. We describe RNA quality control, sample labeling, and array hybridization and scanning.

Tags

DNA MicroarraysSample Quality ControlArray HybridizationScanningGene ActivitiesNervous SystemDevelopment StagesPhysiological StatesPathological StatesTherapy ResponseExperimental TestingHigh Quality RNAAmplificationHybridization ProtocolsAgilent’s 2100 BioanalyzerLab-on-a-chip TechnologyReverse TranscriptionCDNAIn Vitro TranscriptionLabeled CRNADual-color Labeling KitCy3Cy5Agilent’s 4×44 K Arrays

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Bacterial Gene Expression Analysis Using Microarrays

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

Fabrication and Use of MicroEnvironment microArrays (MEArrays)

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

Do’s and Don’ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Read Article