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Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut
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Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut
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09:54 min
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February 14, 2011
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Summary
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Lyme disease research studies often require generation of ticks infected with the pathogen Borrelia burgdorferi, a process that typically takes several weeks. Here we demonstrate a microinjection-based tick infection procedure that can be accomplished within hours. We also demonstrate an immunofluorescence method for in situ localization of B. burgdorferi within ticks.
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Kariu, T., Coleman, A. S., Anderson, J. F., Pal, U. Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut. J. Vis. Exp. (48), e2544, doi:10.3791/2544 (2011).
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