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A Simple Way to Measure Ethanol Sensitivity in Flies
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Neurowissenschaften
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JoVE Journal Neurowissenschaften
A Simple Way to Measure Ethanol Sensitivity in Flies

A Simple Way to Measure Ethanol Sensitivity in Flies

DOI:
10.3791/2541-v

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11:15 min

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February 19, 2011

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1Department of Psychiatry, University of Texas Southwestern Medical Center

Kapitel

  • 00:02Titel
  • 00:47Materials
  • 01:40Creating the exposure chamber
  • 02:38Exposing the flies
  • 03:57Monitoring mobility
  • 05:29Rules for determining mobility
  • 06:39Calculating ST50
  • 07:59Ergebnisse
  • 09:37Tolerance

Summary

Automatische Übersetzung

Automatische Übersetzung

A simple assay to measure the sedating effects of ethanol on Drosophila flies, based on the loss of righting reflex, is described.

Tags

Ethanol SensitivityFliesHyperactivitySedationEthanol PreferenceGenetikAlcohol ResponsesDrinkingIntoxicating EffectsAssayAcute SensitivityEthanol ToleranceRepeat ExposureLaboratory Tools

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