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Journal
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Immunologie und Infektion
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使用泛病毒基因芯片检测(Virochip)屏幕病毒病原体的临床标本
JoVE Journal
Immunologie und Infektion
This content is Free Access.
JoVE Journal
Immunologie und Infektion
Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
Please note that all translations are automatically generated.
Click here for the English version.
使用泛病毒基因芯片检测(Virochip)屏幕病毒病原体的临床标本
DOI:
10.3791/2536-v
•
13:45 min
•
April 27, 2011
•
Eunice C. Chen
,
Steve A. Miller
,
Joseph L. DeRisi
2
,
Charles Y. Chiu
2
1
Department of Laboratory Medicine
,
University of California, San Francisco
,
2
Division of Infectious Diseases
,
University of California, San Francisco
Kapitel
00:05
Titel
01:00
Introduction
02:23
Nucleic Acid Extraction
03:45
Reverse Transcription and 2nd-strand cDNA Synthesis (“Round A”)
05:36
PCR Amplification of Randomly Primed cDNA
06:40
Cy3 Fluorescent Dye Incorporation
08:02
Virochip Microarray Hybridization, Scanning and Analysis
10:34
Virus Detection Using Virochip Microarray
12:15
Conclusion
Summary
Automatische Übersetzung
English (Original)
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Automatische Übersetzung
Virochip是一个泛病毒的芯片设计,同时检测保守序列同源性的基础上所有已知的病毒,以及新颖的病毒。在这里,我们演示了如何运行的Virochip检测,分析存在的已知和未知病毒的临床样本。
Tags
Pan-Viral Microarray Assay
Virochip
Clinical Samples
Viral Pathogens
Viral Diagnosis
Sequence Diversity
Novel Viral Pathogens
SARS Coronavirus
H1N1 Influenza Virus
Traditional Methods
Pan-viral Microarray Platform
Conserved Sequence Homology
Respiratory Infections
Unexplained Critical Illness
Conventional Clinical Testing
Novel Viruses
Rhinovirus Clade
XMRV
Avian Bornavirus
Cardiovirus
Agilent Microarray Platform
GenBank
Article
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