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Crystallization of Membrane Proteins in Lipidic Mesophases
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March 28, 2011
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The protocols describe the essential steps for obtaining diffraction quality crystals of a membrane protein starting from reconstitution of the protein in a lipidic cubic phase (LCP), finding initial conditions with LCP-FRAP pre-crystallization assays, setting up LCP crystallization trials and harvesting crystals.
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CrystallizationMembrane ProteinsLipidic MesophasesSignal TransductionTransportEnergy TransformationsMalfunctionsDiseasesStructural UnderstandingFunctional UnderstandingSolubilizationDiffraction Quality CrystalsIn Meso CrystallizationNative-like Membrane EnvironmentSolvent ContentOrderingTraditional CrystallizationDetergent SolutionsLipid Phase BehaviorViscous Mesophase MaterialsLCP (Lipid Cubic Phase)Syringe MixerReconstitutionLipid BilayerAssay PlateCrystallization Plate
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Liu, W., Cherezov, V. Crystallization of Membrane Proteins in Lipidic Mesophases. J. Vis. Exp. (49), e2501, doi:10.3791/2501 (2011).
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