This content is Free Access.
Crystallization of Membrane Proteins in Lipidic Mesophases
Kapitel
- 00:05Titel
- 01:24Introduction
- 02:09Protein Reconstitution in LCP (Lipid Cubic Phase)
- 03:42Lipid Cubic Phase – Fluorescence Recovery After Photobleaching (LCP-FRAP) Pre-Crystallization Assay
- 06:55Preparation and Harvest of LCP Crystals
- 09:25Representative Results: Crystallization of Human Beta 2 Adrenergic G Protein-Coupled Receptor
- 11:06Conclusion
The protocols describe the essential steps for obtaining diffraction quality crystals of a membrane protein starting from reconstitution of the protein in a lipidic cubic phase (LCP), finding initial conditions with LCP-FRAP pre-crystallization assays, setting up LCP crystallization trials and harvesting crystals.
Tags
CrystallizationMembrane ProteinsLipidic MesophasesSignal TransductionTransportEnergy TransformationsMalfunctionsDiseasesStructural UnderstandingFunctional UnderstandingSolubilizationDiffraction Quality CrystalsIn Meso CrystallizationNative-like Membrane EnvironmentSolvent ContentOrderingTraditional CrystallizationDetergent SolutionsLipid Phase BehaviorViscous Mesophase MaterialsLCP (Lipid Cubic Phase)Syringe MixerReconstitutionLipid BilayerAssay PlateCrystallization Plate
