We developed an alternant hindlimb unloading model in mice. The primary advantage of our hindlimb unloading tail-ring method over the conventional Morey-Holton tail-traction technique is a simple straightforward procedure that minimizes stress upon the animal.
Ferreira, J. A., Crissey, J. M., Brown, M. An Alternant Method to the Traditional NASA Hindlimb Unloading Model in Mice. J. Vis. Exp. (49), e2467, doi:10.3791/2467 (2011).